Research Technician II - Tang Lab

Dr. Jonathan Tang's lab at Seattle Children’s Research Institute is seeking a highly motivated and skilled Research Technician to join our team. This role involves conducting cutting-edge research focused on molecular biology, cell culture, virus production, mouse handling, and FACS analysis. The ideal candidate will have a strong background in biological research, a proactive approach to problem-solving, and a keen interest in contributing to pediatric health advancements.

Key Responsibilities:

- Perform a variety of molecular biology procedures including PCR, gel electrophoresis, DNA sub-cloning, Gibson assembly, and next-generation sequencing. 

- Maintain and culture cell lines, specifically human 293T cells and HeLa cells, under sterile conditions. 

- Produce and titrate viral vectors such as Adeno-associated virus and Mouse Moloney Leukemia Virus.

- Handle mice for genetic studies, including ear clipping, tail snipping, and DNA genotyping.

- Conduct FACS analysis to sort and analyze cell populations based on specific molecular characteristics.

- Analyze experimental data and contribute to the preparation of scientific papers and grant applications.

- Maintain lab equipment and order supplies as necessary to ensure a functional and efficient research environment.

- Collaborate with team members to support various projects and ensure that all protocols and procedures comply with institutional safety and ethical standards.

Required Education and Experience:
Bachelor's degree in Molecular Biology, Biochemistry, Genetics, or a related field. 
At least one (1) year of experience in a laboratory/research setting and/or experience with molecular biology and biochemistry techniques.

Preferred:
Master's degree

At least 2 years of laboratory experience in molecular biology, cell culture techniques, and FACS.

Proven expertise in viral vector production and handling laboratory mice is highly desirable.

Strong organizational skills and the ability to work both independently and as part of a team.

Excellent written and verbal communication skills.

Dedication to high-quality research and a strong commitment to advancing pediatric health.